qPCR assay for the detection of Mycoplasma bovis and Staphylococcus aureus DNA in samples from Cattle, Small ruminants.
Details
INgene® q M. bovis and S. aureus MULTIPLEX is a real-time PCR kit for the detection of M. bovis and S. aureus DNA in biological samples from Cattle, Small ruminants. The assay is optimized for samples like reproductive secretions, preputial, vaginal/endocervical flushings, semen, placenta, fetal tissues and strains/vaccines. The kit consists on a multiplex real-time PCR in one-well format, detecting M. bovis in FAM channel and S. aureus in HEX/VIC channel. In addition, the mix includes and Endogenous control (EC) that is amplified in Cy5, allowing identification of false negatives due to poor extraction and/or PCR inhibition.
Main assay benefits are:- Easy to use format, minimun preparation time
- Harmonized thermal profile across most INgene® q kits
- Broadly compatible with available PCR instruments
- Optional quantification with the positive control
- Validated with clinical samples
- Extended shelf life: 18 months
- 100 rxn format
Kit Specifications
SKU
R.10.XMA.K.5/100
Test principle
qPCR
No. of tests
100
Min no. of samples
100
Max no. of samples
100
Detection
Mycoplasma bovis and Staphylococcus aureus DNA in samples from Cattle, Small ruminants.
Shelf Life (months)
18
Storage
-18°C
Technology
PCR